Composite
Part:BBa_K1935013
Designed by: Emilie Gounin Group: iGEM16_Bordeaux (2016-10-13)
White Collar Complex under control of C.elegans's promoter (pUNC119::WC1::P2A::WC2)
This construction was made to create the White Collar Complex. This one will be injected in Caenorhabditis elegans.
See BBa_K1935002 for details on UNC-119 promoter
See BBa_K1935003 for details on WC-1
See BBa_K1537016 for details on 2A peptide
See BBa_K1935005 for details on WC-2
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 4229
Illegal BglII site found at 4865
Illegal BamHI site found at 4510
Illegal BamHI site found at 5669
Illegal BamHI site found at 5705
Illegal BamHI site found at 6479 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 6895
Illegal NgoMIV site found at 6954
Illegal AgeI site found at 6501 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3792
Illegal BsaI site found at 4136
Illegal BsaI site found at 4589
Illegal BsaI site found at 6046
Illegal BsaI.rc site found at 6414
Illegal SapI.rc site found at 4731
Illegal SapI.rc site found at 5030
Illegal SapI.rc site found at 6296
[edit]
Categories
Parameters
None |